I’d break the drive up from rome to Tuscany with 2 stops:
Orvieto - duomo is second only to Florence’s.
Civita di bargnoregio - please, please, please, don’t look at pictures beforehand on line. Just trust me and go there to visit the citadel. I guarantee you won’t be disappointed.
One word of advice, hire a car with snow tires as there’s a strong chance of snow given how mountainous everywhere in Italy is.